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修复剂调控铅镉污染棉田对土壤微生物多样性的影响
安梦洁, 王开勇, 王海江, 鄂玉联, 孟春梅
石河子大学农学院
摘要:
为探讨重金属铅(Pb)、镉(Cd)污染下棉粕腐植酸和聚丙烯酸钾修复剂对土壤微生物多样性的影响,采用田间桶栽试验,进行了棉粕腐植酸、聚丙烯酸钾以及两者复合施用修复污染棉田土壤的研究,通过高通量测序分析了土壤微生物多样性的变化,并利用傅里叶红外光谱进行了土壤化学结构的修复响应分析。结果表明:与未施用修复剂处理相比,施用棉粕腐植酸和聚丙烯酸钾减少了表层土壤Pb、Cd含量,其中两者复合处理可分别减少62.6% 和52.3%;改变了土壤微环境进而影响了土壤微生物多样性,不同处理共发现土壤细菌25 个门,69个纲,149个目,273个科,442个属,其中优势菌门主要为变形菌门、酸杆菌门、芽单胞菌门和放线菌门。聚丙烯酸钾处理增加了土壤中变形菌门的相对丰度,棉粕腐植酸和复合修复处理增加了酸杆菌门和芽单胞菌门的相对丰度。由此可见,铅镉复合污染土壤中微生物多样性丰富,优势菌群相对稳定,聚丙烯酸钾和棉粕腐植酸通过改变土壤微环境进而影响菌群丰度。
关键词:  铅镉污染  腐植酸  聚丙烯酸钾  高通量测序  傅里叶红外光谱
DOI:10.13758/j.cnki.tr.2019.03.018
分类号:X53
基金项目:国家国际科技合作专项(2015DFA11660)、国家科技支撑计划项目(2014BAC14B030-2)、国家重点研发计划项目(2016YFC 0501406)、国家自然科学基金项目(31560169)和石河子大学国际科技合作项目(GJHZ201802)资助。
Effects of Remediation on Microbial Diversity of Soil Polluted by Pb and Cd
AN Mengjie, WANG Kaiyong, WANG Haijiang, E Yulian, MENG Chunmei
AgricuHural College, Shihezi University
Abstract:
A tub experiment was conducted to investigate the effects of humic acid and potassium polyacrylate on soil biological diversity under heavy metal lead (Pb) and cadmium (Cd) pollution, in which 600 mg/kg of Pb and 1.8 mg/kg of Cd were added into all tubs (T2), then 10 g/kg of cottonseed meal humic acid (T4), 2 g/kg of polyacrylic acid potassium (T6), the combination of T4 and T6 (T8) were added into the tubs, respectively. Soil microbial biodiversity was analyzed by high-throughput sequencing and the remediation response was studied by Fourier transform infrared spectrum. The results showed that the contents of Cd and Pb in the topsoil were reduced in the treatments of T4, T6 and T8, the maximum reduction occurred in T8, which were 62.6% for Cd and 52.3% for Pb, respectively, thus, it changed soil micro-environment and thus affected soil microorganisms. The bacteria in the four treatments covered 25 phyla, 69 classes, 149 orders, 273 families and 442 genera, and Proteobacteria, Acidobacteria, Gemmatimonadetes and Actinobacteria were the dominant bacteria. T6 increased the total number of Proteobacteria, while T4 and T8 increased Acidobacteria and Gemmatimonadetes. It can be concluded that the microbial diversity was very rich and the dominant bacterial community was relatively stable in Cd and Pb polluted soil. Potassium polyacrylate and cottonseed meal humic acid affected composition abundance of bacterial flora by changing soil microenvironment.
Key words:  Lead and cadmium pollution  Humic acid  Potassium polyacrylate  high-throughput sequencing  Fourier transform infrared spectrum

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