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一株高效解钾脱硅真菌的筛选、鉴定及培养条件优化
张红芳,何 刚,吴绿英,孙德四,陈 晔
九江学院药学与生命科学学院
摘要:
为挖掘植物内生真菌功能活性菌株资源,从钾矿区优势蕨类植物——乌蕨(Stenoloma chusanum)中筛选得到一株高效解钾脱硅菌株WJ007。通过形态学特征观察、rDNA ITS序列分析以及系统发育关系比较等研究,确定该菌株为小刺青霉Penicillium spinulosum。通过单因素试验与正交试验对WJ007菌株解钾、脱硅培养条件进行优化,结果显示:WJ007菌株脱硅最优培养条件为葡萄糖作碳源、酵母膏作氮源、碳氮比60∶1、初始pH 6,在该条件下培养液中可溶性硅含量为8.74 mg/L;WJ007菌株解钾最优培养条件为葡萄糖作碳源、蛋白胨作氮源、碳氮比60∶1、初始pH 7,在该条件下培养液中可溶性钾含量为154.44 mg/L。通过对WJ007菌株解钾脱硅发酵条件的优化,可为植物内生真菌资源开发利用提供依据。
关键词:  内生真菌  解钾  脱硅  筛选  鉴定  培养条件
DOI:10.13758/j.cnki.tr.2018.05.012
分类号:S154. 39
基金项目:国家自然科学基金项目(31360064;51264014)和江西省自然科学基金项目(20171BAB204007)资助。
Screening, Identification and Cultivated Condition Optimization of High Efficiency Potassium Solubilization and Desilication Fungus Strain
ZHANG Hongfang1, HE Gang2, WU Lvying2, SUN Desi2, CHEN Ye2
1.School of Pharmacy and Life Sciences,Jiujiang University;2.School of Pharmacy and Life Sciences, Jiujiang University
Abstract:
In order to excavate the functional endophyte fungi resources, a fungus strain, named WJ007 with high-efficiency potassium solubilization and desilication was screened from Stenoloma chusanum, it was identified as Penicillium spinulosum based on morphological characteristics, rDNA ITS sequence and phylogenetic relationships analyses. Its optimal culture conditions obtained by single factor and orthogonal experimens for desiliconization were glucose as carbon source, yeast extract as nitrogen source, carbon and nitrogen ratio of 60∶1, initial pH 6, and culture soluble silicon content in liquid is 8.74 mg/L under these conditions; while its optimal culture conditions for potassium solubilization were glucose as carbon source, protein peptone as nitrogen source, carbon and nitrogen ratio of 60∶1, initial pH 7, and soluble potassium contents in culture solution is 154.44 mg/L under these conditions. It is significant for the development and utilization of plant endophytic fungi to optimize fermentation conditions of potassium solubilization and desiliconization for WJ007.
Key words:  Endophytic fungi  Potassium-solubilization  Desilication  Isolation  Identification  Fermentation condition

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