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拟南芥14-3-3蛋白GRF9调控番茄根系响应水分胁迫的生理机制
章丽丽, 李光杰, 陆玉芳, 施卫明
土壤与农业可持续发展国家重点实验室中国科学院南京土壤研究所
摘要:
采用35S启动子控制Arabidopsis General Regulatory Factor 9 (AtGRF9) 在两个转基因番茄株系(E2,E7)中高效表达,以野生型番茄WT、转基因番茄E2和E7三个株系为试验材料,在水培条件下用20% 聚乙二醇(PEG 6000)模拟干旱胁迫,探究了拟南芥14-3-3蛋白GRF9能否增强番茄根系响应水分胁迫的能力。结果表明:①在干旱胁迫下,野生型番茄和转基因番茄的根系形态指标均受到不同程度的抑制,WT、E2和E7三个番茄材料相对总根长的受抑制程度分别为43%、28%、30%,相对根表面积的受抑制程度分别为46%、33%、35%,相对根体积的受抑制程度分别为47%、32%、29%,相对根直径的受抑制程度分别为29%、21%、22%。②在响应干旱胁迫时,转基因番茄根系蔗糖含量比野生型番茄高20%,根系干物质量比野生型番茄高23%。③在干旱胁迫时,转基因番茄根系质膜H+-ATPase酶活性较高,具有较强的分泌质子的能力,其根系泌酸量比野生型番茄高35%。因此,GRF9能够促进番茄根系蔗糖含量的增加和干物质的累积、增强根系分泌质子的能力,这对于转基因番茄根系在总根长以及根表面积、根体积和根直径的生长以响应干旱胁迫的过程中具有重要作用。
关键词:  番茄  GRF9  根系形态  质子分泌  干旱胁迫
DOI:10.13758/j.cnki.tr.2020.01.011
分类号:Q945.12
基金项目:江苏省青年基金项目(BK20151053) 和中国科学院南京土壤研究所知识创新工程领域前沿项目(ISSASIP1604)资助。
Involvement of Arabidopsis GRF9 in Tomato Root Growth and Response Under Polyethylene Glycol Induced Water Stress
Zhang Lili, Li Guangjie, Lu Yufang, Shi Weiming
State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science,Chinese Academy of Sciences
Abstract:
In order to improve water stress tolerance of tomato (Solanum lycopersicum) plants, an expression vector containing an arabidopsis 14-3-3 protein, Arabidopsis General Regulatory Factor 9 (AtGRF9) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into tomato plants. Tomato wild-type (WT), two lines of GRF9-overexpressing tomato plants (E2, E7) were treated with 20% polyethylene glycol (PEG6000) to induce water stress under hydroponic culture conditions. Results showed: 1) The degree of water stress tolerance of transgenic tomato plants was found to be significantly greater than that of wild-type tomato plants as measured by root architecture development. The relative inhibition ratio of total root length of three lines tomato plants (WT, E2, E7) was 43%, 28% and 30%, respectively; the relative inhibition ratio of root surface area of three lines tomato plants (WT, E2, E7) was 46%, 33% and 35%, respectively; the relative inhibition ratio of root volume of three lines tomato plants (WT, E2, E7) was 47%, 32% and 29%, respectively; the relative inhibition ratio of root diameter of three lines tomato plants (WT, E2, E7) was 29%, 21% and 22%, respectively. 2) GRF9 favored the accumulation of sucrose in transgenic tomato (E2, E7) roots, and the root dry weight was 23% higher than that of WT. 3) In addition, GRF9 enhanced the activity of plasma membrane H+-ATPase in transgenic tomato (E2,E7) roots, and the root proton secretion was 35% higher than that of WT. Taken together, all the results indicated that under PEG-induced water stress, GRF9 is involved in enhancing proton secretion and accumulating more sucrose in the root to guarantee greater root architecture development on total root length, root surfaces area, root volume and root diameter. Therefore, Arabidopsis GRF9 plays an important role for tomato plants response to water stress.
Key words:  Solanum lycopersicum  GRF9  Root architecture  Proton secretion  Water stress

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