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铜胁迫对抗虫转基因水稻根际土壤微生物的影响
王宵宵1, 钟文辉1, 李磊1, 洪鑫1, 刘标2, 韩成1
1.南京师范大学;2.环境保护部南京环境科学研究所
摘要:
根际土壤微生物群落是联系土壤环境与作物生长的重要纽带,也是转基因作物环境安全评价的主要指标,而Cu胁迫对转基因水稻根际土壤微生物的影响目前尚不清楚。本研究基于盆栽试验,采用高通量测序等技术研究Cu胁迫(Cu含量100 mg/kg)对抗虫转基因水稻华恢1号(HH)及其亲本非转基因水稻明恢63(MH)农艺性状及成熟期根际土壤微生物的影响,并以不施加Cu胁迫处理为对照。结果显示:Cu胁迫显著降低了水稻株高、生物量及产量;Cu胁迫改变了水稻根际土壤总氮、铵态氮含量及氧化还原电位值,而种植转基因水稻仅降低了根际土壤氧化还原电位值;Cu胁迫没有影响水稻根际土壤细菌丰度,但降低了细菌群落Alpha-多样性,改变了水稻根际土壤细菌群落组成和群落结构;相同Cu含量胁迫下,HH和MH水稻生长指标及根际土壤细菌群落结构及组成差异较小。上述研究表明,Cu胁迫抑制了水稻农艺性状及根际土壤细菌群落,但种植抗虫转基因水稻没有影响水稻植株及根际土壤细菌群落对Cu胁迫的抗性。
关键词:  铜胁迫  细菌群落  转基因水稻  根际土壤  环境安全评价
DOI:10.13758/j.cnki.tr.2020.01.017
分类号:S154.3;Q938.1
基金项目:农业部转基因生物新品种培育重大专项项目(2016ZX08012005)资助。
Effects of Cu Stress on Rhizosphere Soil Microorganisms of Insect-resistant Transgenic Rice
Wang Xiaoxiao1, Zhong Wenhui1, Li Lei1, Hong Xin1, Liu Biao2, Han Cheng1
1.Nanjing Normal University;2.Nanjing Institute of Environmental Sciences,MEP
Abstract:
Soil environment and crop growth can be connected in rhizosphere soil, which is also the main indicator of the environmental safety assessment of the transgenic rice, but the effect of Cu stress on rhizosphere microorganisms of insect- resistant transgenic rice is still unclear. A pot experiment was conducted in this study and the Illumina Hiseq pyrosequencing technology was carried out to investigate the rhizosphere microbial community of insect-resistant transgenic rice Huahui1 (HH) and its non-transgenic parent line Minghui63 (MH) with or without 100 mg/kg Cu stress (abbreviated as Cu100 and Cu0, respectively). Results showed that Cu stress significantly reduced rice height, biomass and yield, and changed total N content, ammonium content and redox potential value in rice rhizosphere soil. Cu stress did not change the bacterial community abundance, but altered the composition and structure of bacterial community and decreased the Alpha-diversity of bacterial community. No effect on the rice growth and composition and structure of bacterial community was observed between HH and MH varieties with or without Cu stress. These findings indicate that Cu stress can suppress the agronomic traits of rice and the rhizosphere bacterial community, but planting insect-resistant transgenic rice does not change the resistance of rice and rhizosphere bacteria to Cu stress.
Key words:  Cu  Bacterial community  Transgenic rice  Rhizosphere soil  Environmental safety

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