| 摘要: |
| 通过田间试验研究了不同剂量磷灰石(10、20、30、50 g/kg，编号分别为LH1、LH2、LH3和LH4)与黑麦联合作用对Cu污染土壤的修复效果，重点考察了磷灰石对黑麦生物量及Cu吸收、土壤pH及Cu形态转化、土壤酶活性、植物生理指标等的影响。试验结果表明，磷灰石显著提高了土壤pH、黑麦生物量及其Cu吸收量，显著降低了黑麦地上部分及根系Cu含量，其中LH3处理黑麦Cu吸收量最高，达到对照处理的33.38倍；与对照相比，LH1、LH2、LH3和LH4处理土壤可交换态Cu含量显著降低了37.0%、58.9%、89.0% 和83.3%，碳酸盐结合态Cu含量显著增加了46.6%、63.1%、78.0% 和93.3%；与对照相比，LH2、LH3和LH4处理显著提高了土壤磷酸酶、过氧化氢酶和脲酶活性，显著提高了黑麦SOD和POD酶活性，显著降低了黑麦MDA含量。综合而言，施用30 g/kg的磷灰石并种植黑麦能够很好地修复Cu污染土壤。 |
| 关键词: 磷灰石 黑麦 修复 铜 土壤酶活性 植物生理指标 |
| DOI：10.13758/j.cnki.tr.2019.02.017 |
| 分类号:X53 |
| 基金项目:国家自然科学基金项目(31800361)；2019年河南省科学院基本科研业务费项目(190605019)；2017年河南省科学院基本科研业务费项目 (铜尾矿耐性菌株的筛选及其对黑麦草生长影响研究)；2017年河南省科学院预先研究项目 (改良剂对铜镉污染土壤的修复及其对微生物多样性的影响研究)；2016年河南省科技攻关项目 (162102110164)；河南省科学院重大科研聚焦专项(190108003)；2012年河南省重大科技专项(121100110100)和2018年河南省科技开放合作项目(182106000053)资助。 |
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| Study on Apatite Combined Rye on Remediation of Cu Contaminated Soil |
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DU Zhimin1, GUO Xuebai2, ZHEN Jing1, LUO Hongji3, ZHOU Jing4, WANG Jiwen1, YUE Dandan1, GONG Tao1, YANG Wenling1, CHEN Guocan1
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1.Institute of Biology Co. Ltd., Henan Academy of Sciences;2.Zhengzhou Technology and Business University;3.Sichuan Changhong Green Environmental Science and Technology Co. Ltd.;4.Institute of Soil Science, Chinese Academy of Sciences
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| Abstract: |
| A field experiment was conducted to test the remediation effects in a Cu contaminated soil by applying apatite (LH1, LH2, LH3 and LH4, dosages of 10, 20, 30 and 50 g/kg, respectively) and rye (Secale cereale), the biomass and Cu accumulation of rye, soil pH and Cu speciation, enzyme activities, and plant physiological indexes were measured. The results showed that apatite significantly increased soil pH, biomass and Cu accumulation of rye, but decreased Cu concentrations in shoots and roots of rye. Compared to control, LH3 increased Cu absorption of rye by 33.38 times of control, which was the highest treatment. Compared to control, LH1, LH2, LH3 and LH4 significantly decreased soil exchangeable Cu by 37.0%, 58.9%, 89.0% and 83.3%, while increased carbonate-bound Cu by 46.6%, 63.1%, 78.0% and 93.3%, respectively. LH2, LH3 and LH4 increased soil phosphatase, calatase, and urese activities, and increased SOD and POD activities of rye, but decreased MDA content of rye. In general, combining apatite of 30 g/kg with rye could remediate effectively Cu contaminated soil. |
| Key words: Apatite Rye Remediation Cu Soil enzyme activities Plant physiological index |
