Ubiquitous existence of antibiotic resistant pathogens (ARP) in the agricultural land and vegetation system has posed great threat against public health and environmental safety, making it essential to develop targeted controlling technologies. In this work, biochar and bacteriophage combined technology was investigated for its effectiveness in targeted inactivating the ARP in the soil-lettuce system. Biochar and polyvalent phage YSZ 5K were applied to the ARP (tetracycline resistant Escherichia coli K12 and chloramphenicol resistant Pseudomonas aeruginosa PAO1) contaminated soil. The residual K12 and PAO1 counts and antibiotic resistance genes (tetW and ampC) abundances were determined after 60 days of incubation. The results showed that the combined technology was proved most effective, followed by sole application of biochar, sole inoculation of YSZ 5K, and the control. For the combined treatment, the counts of K12 / PAO1 decreased by 2.1-3.1 magnitudes and the abundances oftetW/ampC decreased by 2.2-3.3 magnitudes in the soil and lettuce leaves, respectively. In addition, the structural and functioning diversity of soil microbial community was improved significantly after the combined treatment. The results obtained here demonstrate the combined application of biochar and polyvalent phage YSZ 5K an environmentally-friendly technology that could effectively decrease the ARP in the soil-lettuce system.