A pot experiment was conducted with static box-gas chromatographic method to investigate the effects of different combined application of organic and inorganic nitrogen (N) fertilizers on soil N₂O emissions from lettuce (Lactuca sativa L.) field. There were six treatments, including 1) no fertilizer (CK), 2) no N fertilizer (PK), 3) pure fertilizer (NPK), 4) organic and inorganic fertilizer 1 (20% pig manure N + 80% chemical fertilizer N, NPKM1), 5) organic and inorganic fertilizer 2 (20% biogas manure N + 80% chemical fertilizer N, NPKM2), and 6) organic and inorganic fertilizer 3 (20% pig manure composting N + 80% chemical fertilizer N, NPKM3). Results showed that N₂O emission peaks appeared after fertilizer application during lettuce growing season, and the peak time and intensities were differred among different treatments. N₂O accumulation emission gradually increased along with the growth period. Soil average N₂O emission flux and N₂O accumulation emission were 0.10–0.25 mg/(m²?h) and 1.37–3.42 kg/hm², and in the order of NPK > NPKM2 > PK> NPKM1 > NPKM3 > CK. Soil N₂O emission factor was 0.13%–0.68%, and in the order of NPK > NPKM2 > NPKM1 > NPKM3. Compared with NPK treatment, NPKM1, NPKM2 and NPKM3 treatments reduced soil N₂O accumulation emission by 48.08%, 25.75% and 48.30%, and increased lettuce yield by 48.66%, 22.13% and 53.76%, respectively. In conclusion, soil N₂O emission from lettuce field was promoted by pure fertilizer application, while decreased by the addition of different organic N fertilizers with higher production, especatilly in pig manure (composting or not) treatments. Therefore, combined application of organic and inorganic N fertilizers is an effective way to reduce N₂O and N loss by emission in vegetable field.