The temporal and spatial variation characteristics of soil organic matter (SOM) content in tobacco planting fields in Jingxi and their relationship with soil nutrient contents and tobacco-rice rotation years were analyzed. The results showed that: 1) SOM contents in 2006, 2012 and 2017 were all generally high and rich, with the mean value of 35.72, 35.80 and 38.84 g/kg, respectively. There were no deficient level soil samples, and the low-level soil samples in 2012 were only 5.30%. Geostatistics showed that the overall appearance was high and the above spots were more abundant. 2) SOM content increased with time, and SOM content in 2017 was significantly higher than those in 2006 and 2012. 3) Regression analysis between SOM and other soil nutrients in 2017 showed that total nitrogen, hydrolyzable alkali nitrogen had higher positive correlation with SOM, and the coefficient of determination was above 0.5000.