Based on the methods of classical statistics and geostatistics, the spatiotemporal variation of soil organic matter (SOM) and total nitrogen (TN) from 2000 to 2015 in tobacco-planting area of Xiangxi were studied in order to provide scientific basis for reasonable application of organic and nitrogen fertilizers. The results showed that from 2000 to 2015 average SOM and TN increased by 5.03 g/kg (21.39%) and 0.31 g/kg (21.99%), respectively. For SOM and TN, their minima decreased while variation coefficients, maxima and ranges increased. In the past 15 years, the normalized Z values of Moran's I indexes of SOM and TN decreased, nugget effects increased; the “low” and “suitable” grade areas of SOM and TN decreased by 3.42%, 37.73% and 2.04% , 44.43%, respectively; while the “high” and “extremely high” grade areas of SOM and TN increased by 32.21%, 8.94% and 35.67%, 10.80%, respectively. In general, SOM and TN contents and their variations increased significantly in tobacco growing area of Xiangxi, the spatial autocorrelation of SOM and TN were weakened and the random variabilities were enhanced, and SOM showed an extremely significant positive linear correlation with TN, the mineralization and decomposition of soil SOM is the main source of TN.