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不同形态硒对金针菇吸收和富集硒的影响
万亚男, 罗 章, 王晓芳, 王 琪, 余 垚, 郭岩彬, 李花粉
农田土壤污染防控与修复北京市重点实验室,中国农业大学资源与环境学院
摘要:
通过基质栽培试验,研究了硒酸钠、亚硒酸钠和硒代蛋氨酸(0 ~ 0.8 mg/kg)对金针菇(Flammulina velutipes)吸收和富集硒的影响。结果表明,外源硒的添加显著增加了金针菇原基和子实体的硒含量,且硒含量均随硒添加浓度的上升而显著增加。在相同硒浓度水平下,3种外源硒对金针菇子实体硒含量的增加效果为亚硒酸钠>硒代蛋氨酸>硒酸钠:施硒量为0.8 mg/kg时,3种硒处理下金针菇子实体的硒含量分别为0.24、0.12和0.08 mg/kg。此外,亚硒酸钠处理下金针菇子实体和原基的硒回收率大于硒代蛋氨酸和硒酸钠处理。且随着硒添加浓度的增加,硒代蛋氨酸和硒酸钠处理下子实体硒的回收率显著下降:分别从14.28%逐渐降低到6.33% 和从13.67% 减少到3.70%;而添加硒酸钠时,硒的回收率随添加浓度的增加无显著变化。
关键词:  金针菇  硒酸钠  亚硒酸钠  硒代蛋氨酸  硒回收率
DOI:10.13758/j.cnki.tr.2018.06.019
分类号:S646.9
基金项目:中央高校基本科研业务费(2018TC031)和公益性行业(农业)科研专项(201303106)资助。
Effect of Selenate, Selenite and Selenomethionine on Selenium Accumulation in Flammulina velutipes
WAN Yanan, LUO Zhang, WANG Xiaofang, WANG Qi, YU Yao, GUO Yanbin, LI Huafen
Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University
Abstract:
The effects of basal-applied selenate, selenite and selenomethionine (SeMet) (0 – 0.8 mg/kg) on selenium accumulation in Flammulina velutipes were studied in soil-less culture. Results showed that Se contents in Flammulina velutipes fruiting body and base increased significantly with the increase of Se addition. The application of selenite showed better effect than SeMet and selenate in increasing Se content in Flammulina velutipes. For the substrate amended with 0.8 mg/kg selenite, selenate and SeMet, the Se levels in fruiting body were 0.24, 0.12 and 0.08 mg/kg (fresh mass), respectively. The recovery (fruiting body and root) of Se applied as selenite was greater than SeMet and selenate. What’s more, the recovery of Se in fruiting body in SeMet and selenate decreased significantly from 14.28% to 6.33% and from 13.67% to 3.70%, respectively, with the increase of Se levels in substrate; while no significant difference in the recovery (fruiting body) of Se among different Se levels when applied selenite.
Key words:  Flammulina velutipes  Selenate  Selenite  Selenomethionine  Se recovery

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