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土壤富硒细菌的筛选、鉴定
廖 青, 刘永贤, 邢 颖, 梁潘霞, 潘丽萍, 陈锦平, 江泽普
广西农业科学院农业资源与环境研究所/广西富硒农业研究中心
摘要:
从广西不同富硒区土壤中分离出32株耐硒细菌,通过优化加硒时间、培养时间及加硒浓度进行富硒试验,筛选获得4株富硒能力较强的细菌,进一步通过对4株细菌16S rDNA序列分析及系统发育树分析明确了各菌株的种属关系。经鉴定,YLB1-6为Bacillus cereus,TXB1-10为Sinomonas sp.,TXB2-5为Bacillus thuringiensis,GPB1-5为Achromobacter denitrificans。在最适加硒时间、培养时间及适宜加硒浓度条件下,各菌株的硒转化率分别为:YLB1-6 74.22%,TXB1-10 66.05%,TXB2-5 55.31%,GPB1-5 63.30%。各菌株处理能显著提高土壤可交换态硒含量,对土壤硒起到较强活化作用。土壤富硒细菌为硒转化提供更多的高效微生物载体,并为富硒产品开发研究提供技术支持。
关键词:  富硒细菌  硒转化率  筛选  鉴定
DOI:10.13758/j.cnki.tr.2018.06.023
分类号:Q939.9
基金项目:国家自然科学基金项目(41761052)、广西创新驱动重大专项项目(桂科AA17202019-1、桂科AA17202019-4、桂科AA17202026)、广西重点研发计划项目(桂科AB16380207)、广西农业重点科技计划项目(201604)、广西青年基金项目(2016GXNSFBA380131)、广西富硒特色作物试验站项目(桂TS2016011)、广西农业科学院基本科研业务专项项目(2015YT33、桂农科2017YZ03)和广西农业科学院科技发展基金项目(桂农科2017JM01,桂农科2017JM03,桂农科2018JZ21)资助。
Screening and Identification of Se-enriched Bacteria from Se-rich Soils in Guangxi
LIAO Qing, LIU Yongxian, XING Ying, LIANG Panxia, PAN Liping, CHEN Jinping, JIANG Zepu
Agricultural Resource and Environmental Research Institute, Guangxi Academy of Agricultural Sciences/Selenium Enriched Agriculture Research Center of Guangxi
Abstract:
32 Se-tolerance strains were isolated from Se-rich soils in Guangxi. Se-enriched test were conducted by optimizing the time of adding Na2SeO3, the incubation time and Na2SeO3 concentration, and 4 bacterial strains with strong Se-enriched ability were screened out. Furthermore, the species of these 4 bacterial strains were determined based on the sequencing of 16S rDNA and phylogenetic analysis as Bacillus cereus (YLB1-6), Sinomonas sp. (TXB1-10), Bacillus thuringiensis (TXB2-5) and Achromobacter denitrificans (GPB1-5), respectively. Under the condition of the optimum time of adding Na2SeO3, the suitable incubation time and optimal Na2SeO3 concentration, the ratio of Se transformation of each strain was as follow: YLB1-6 74.22%, TXB1-10 66.05%, TXB2-5 55.31%, GPB1-5 63.30%. Furthermore, all strains treatments could significantly increase the exchangeable Se contents and have strong activating effect on soil Se. Soil Se-enriched bacteria provides more efficient microorganism carrier for Se transformation and provide technical support for Se-enriched products development.
Key words:  Se-enriched bacteria  Ratio of Se transformation  Screening  Identification

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