Eight maize varieties with different genetic backgrounds were planted in two kinds of soils with different selenium contents to study the differences of agronomic characters, yield and selenium content of maize. The results showed that soil selenium content had increasing effects on plant height, ear position and visible leaf number of maize, the three indexes of maize in selenium-enriched region were higher than those in non-selenium-rich region, but maize yield in selenium-enriched region was not higher than that of non-selenium-rich region. The yields of the tested varieties were 7 822.6 kg/hm² for Kangnongyu 108, 7 302.11 kg/hm² for Huayu 11, 6 940.47 kg/hm² for Shuangyu 919, 6 860.41 kg/hm² for Heshengyu 718, 6 374.98 kg/hm² for Zhengda 999, 5 964.29 kg/hm² for Wanyu 168, 5 889.69 kg/hm² for Endan 804 and 5 692.68 kg/hm² for Xingnongdan No. 3, respectively. Selenium content in soil promoted the selenium content in maize kernels, and selenium content in grains of the tested varieties was in an order of Xingnongdan No.3 > Shuangyu 919 > Wanyu168 > Endan 804 > Kangnongyu 108 > Zhengda 999 > Huayu 11 > Heshengyu 718.