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不同浸提剂处理森林土壤溶解性有机碳含量比较
丁咸庆1, 柏 菁2, 项文化1, 侯红波2, 彭佩钦2
1.中南林业科技大学生命科学与技术学院;2.中南林业科技大学环境科学与工程学院
摘要:
为了解亚热带森林土壤溶解性有机碳(DOC)的特征规律,采用培养离心的方法获取土壤溶液测得DOC含量,对比传统水溶性有机碳 (WSOC) 提取法间的差异。选取湖南大山冲森林公园保存完好的3种亚热带典型次生林地,按10 cm一层采集剖面土壤,采用不同方法提取测定土壤DOC和WSOC含量,分析与土壤理化指标的相关性及方法间的显著性关系。结果表明:①典型森林土壤 DOC或WSOC含量随土壤剖面深度的增加,呈显著下降趋势。培养离心提取测得的土壤DOC含量明显较低,仅0.82 ~ 9.52 mg/kg,超纯水浸提的风干土WSOC含量达10.56 ~ 249.19 mg/kg,而0.5 mol/L K2SO4提取的鲜土WSOC含量达155.70 ~ 576.94 mg/kg,0.5 mol/L K2SO4浸提的干土WSOC含量最高,达158.94 ~ 797.56 mg/kg,含量表现为:DOC<干土超纯水浸提WSOC<鲜土 K2SO4浸提WSOC<干土 K2SO4浸提WSOC;②3种次生林土壤DOC或WSOC含量存在显著差异,不同方法测定的含量均表现为针叶林<常绿阔叶林<落叶林;③培养离心法测得土壤DOC含量与其他测定方法间存在极显著正相关(P<0.01),并且土壤DOC和WSOC均与土壤pH、总有机碳、胡敏酸、富啡酸、全氮、全磷、速效态氮以及微生物生物量碳氮达到极显著相关 (P<0.01),与土壤含水率和容重显著相关(P<0.05)。培养离心法测得土壤DOC含量远低于水溶液提取WSOC含量,该方法下的测得值更接近于原位土壤溶解性有机碳实际值。
关键词:  溶解性有机碳  水溶性有机碳  森林类型  培养离心  测定方法
DOI:10.13758/j.cnki.tr.2020.03.014
分类号:S714;S153.6
基金项目:国家自然科学基金项目(31470629)资助。
Comparison of Dissolved Organic Carbon Contents in Forest Soils Extracted by Different Agents
DING Xianqing1, BAI Jin2, XIANG Wenhua1, HOU Hongbo2, PENG Peiqing2
1.Faculty of Life Science and Technology, Central South University of Forestry and Technology;2.Faculty of Environmental Science and Engineering, Central South University of Forestry and Technology
Abstract:
0-10 cm soil samples were collected under three secondary forests in Dashanchong Forest Park in Hunan, the contents of dissolved organic carbon (DOC) and water-soluble organic carbon (WSOC) were measured with different extraction agents. The results showed that: 1) DOC and WSOC contents were decreased significantly with the increase of soil depth. DOC content measured by cultivation-centrifugation method was the lowest (only 0.82 - 9.52 mg/kg), followed by WSOC content extracted by ultra-pure water in air-dried soils (10.56 - 249.19 mg/kg), WSOC contents extracted by K2SO4 in fresh and air-dried soils were the highest (155.70 - 576.94 mg/kg and 158.94 - 797.56 mg/kg, respectively). The average content was in the order of DOC < WSOC extracted by ultra-pure water in air-dried soils < WSOC extracted by K2SO4 in fresh soils < WSOC extracted by K2SO4 in air-dried soils. 2) Significant differences were found in soil DOC or WSOC contents under three forests, and both DOC and WSOC contents determined by different methods were in the order of coniferous forests < evergreen broad-leaved forests < deciduous forest. 3) Extremely significant positive correlation was found between DOC contents measured by the cultivation-centrifugation method and other methods (P<0.01). DOC or WSOC contents were extremely significantly correlated with soil organic carbon, humic acid, fulvic acid, total nitrogen, total phosphorus, available N, microbial biomass carbon, microbial biomass nitrogen and pH (P<0.01), and significantly correlated with soil bulk density and soil water content (P<0.05). DOC content measured by the cultivation-centrifugation method was far lower than WSOC content extracted by salt solution, however, it was closer to the actual value of in-situ measured soil dissolved organic carbon.
Key words:  Dissolved organic carbon (DOC)  Water-soluble organic carbon (WSOC)  Forest type  Cultivation centrifugation  Determination method

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