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外源蔗糖对高NH4+胁迫下拟南芥碳氮代谢的影响
李 祎, 杨顺瑛, 郝东利, 苏彦华
土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)
摘要:
以拟南芥col-0、amt1.1amt1.3为试验材料,采取基质培养的方法,以常规营养液(4 mmol/L NH4+)处理为对照,设置高NH4+ (20 mmol/L)营养液中分别添加0% 蔗糖(T1)、5% 蔗糖(T2)处理,通过测定植株地上部分的鲜重,叶绿素、游离NH4+、可溶性糖、可溶性蛋白、矿质元素含量及谷氨酰胺合成酶(GS)、谷氨酸脱氢酶(GDH)酶活性等指标,研究外源蔗糖对NH4+胁迫拟南芥碳氮代谢的影响。结果表明:与CK相比,T1处理下,3个拟南芥株系植株生长均受到严重的抑制。鲜重及GS、GDH活性降低,游离NH4+、叶绿素、可溶性糖和可溶性蛋白含量增加;植株的氮、磷、钾、钙含量增加,镁、铁含量减少。其中col-0植株在T1处理下受到的抑制比amt1.1amt1.3植株更为显著。与T1处理相比,T2处理增加了拟南芥植株的鲜重,显著提高了可溶性糖和可溶性蛋白含量,提高了GS和GDH活性;降低了叶绿素和游离NH4+ 的含量,提高了植株的氮、磷、钾、钙、镁含量,降低了植株铁含量,其中,外源蔗糖对col-0植株高NH4+ 毒害的缓解较两个突变体植株更为显著。
关键词:  蔗糖  高NH4+ 胁迫  拟南芥  碳代谢  氮代谢
DOI:10.13758/j.cnki.tr.2020.06.003
分类号:Q945.1;
基金项目:中国科学院南京土壤研究所“一三五”计划和领域前沿项目(ISSASIP1609)资助。
Effect of Exogenous Sucrose on Nitrogen and Carbon Metabolism of Arabidopsis Under High NH4+ Stress
Li Yi, YANG Shunying, HAO Dongli, SU Yanhua
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences
Abstract:
Arabidopsis col-0,amt1.1 and amt1.3 were used as materials. By adding 0% (T1) or 5% (T2) sucrose to the culture solution, the influences of exogenous sucrose on nitrogen and carbon metabolism of three Arabidopsis lines in nutrient solution under 20 mmol/L NH4+ were investigated. Plants grown under 4 mmol/L NH4+ were used as controls (CK). The fresh weight, the contents of chlorophyll, NH4+- N, soluble sugar and protein, the activity of glutamine synthetase (GS) and glutamate dehydrogenase (GDH), the mineral element contents of aboveground parts of Arabidopsis were determined in this study. The results showed that, the growth of three Arabidopsis lines was significantly restrained under 20 mmol/L NH4+ condition (T1) compared with 4 mmol/L NH4+ (CK). With the increasing of NH4+ concentration, the fresh weight and the GS and GDH activities were reduced, but the contents of NH4+, chlorophyll, soluble sugar, soluble proteins, N, P, K and Ca of Arabidopsis were increased, while the contents of Mg and Fe of Arabidopsis were reduced. Compared with amt1.1 and amt1.3, col-0 was more sensitive to high NH4+. Compared with T1 treatment, T2 treatment increased the fresh weight of Arabidopsis, significantly increased the contents of soluble sugar and protein, enhanced the activities of GS and GDH, reduced the contents of ammonium and chlorophyll of Arabidopsis, increased the contents of N, P, K, Ca and Mg but reduced the content of Fe in Arabidopsis. Compared with amt1.1 and amt1.3, exogenous addition of sucrose (T2) showed a better alleviation effect on high NH4+-stress in Col-0.
Key words:  Sucrose  High NH4+ stress  Arabidopsis thaliana  Carbon metabolism  Nitrogen metabolism

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