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水稻生物硝化抑制剂1,9-癸二醇的定量方法优化
张晓楠, 陆玉芳, 杨 婷, 施卫明
中国科学院南京土壤研究所
摘要:
选取水稻生物硝化抑制剂1,9-癸二醇作为研究对象,对比旋转蒸发法和固相萃取法对该物质的回收率(80.17% 和82.97%)及效率,确定固相萃取法为水稻根系分泌物收集液的前处理方法,且该方法具有高效省时的特点。在气相色谱(GC)分析方法的基础上,对衍生化试剂和衍生化条件进行了优化,结果表明,使用N,O-双(三甲基硅基)三氟乙酰胺(BSTFA)作为1,9-癸二醇的衍生化试剂时,衍生化产物具有较高的响应值;衍生化过程中加入200 μl BSTFA,在60 ℃ 条件下反应30 min时,1,9-癸二醇经过GC方法得到的分析效果最好;且本方法仪器的日内精密度为2.18%,日间精密度为3.01%,线性方程为 y = 34.77 x-0.90, r = 0.999 3,最小检出限为 0.05 μg/ml,此方法可为水稻根系分泌物中生物硝化抑制剂1,9-癸二醇的定量研究提供参考。
关键词:  硝化作用  水稻  1,9-癸二醇  生物硝化抑制剂  定量
DOI:10.13758/j.cnki.tr.2020.06.008
分类号:S145.9
基金项目:中国科学院战略性先导科技专项(B类) (XDB15030100)和国家自然科学基金重点项目(31761143015)资助。
Optimized Determination of Biological Nitrification Inhibitor 1,9-decanediol in Rice Root Exudates
ZHANG Xiaonan, LU Yufang, YANG Ting, SHI Weiming
Institute of Soil Science, Chinese Academy of Sciences
Abstract:
The optimized pre-treatment and derivatization methods were developed in this study for the determination of 1,9-decanediol by gas chromatograph in the rice root exudates. Results showed that the C18 SPE column could retain 1,9-decanediol efficiently and its relative recovery rate (82.97%) had no significant difference with that of the rotary evaporation (80.17%). This method is time-saving and fit for concentrating the identified hydrophobic BNI-compound, 1,9-decanediol, in large amount of root exudates solutions. In addition, the use of N,O-bis (trimethylsily) trifluoroacetamide (BSTFA, 200 μl) as derivatization reagent, together with 30 min heating in the oven at 60 ℃, was found to be the most efficient method for stable derivatives and high sensitivity. The linear equation was y = 34.77 x-0.90 (r = 0.999 3); the 1,9-decanediol detection limit of the method was 0.05 μg/ml; and the method exhibited good accuracy (recoveries of 94.42%) and precision (within-day precision of 2.18% and day-to-day precision of 3.01%) which could provide reference for the subsequent analysis of 1,9-decanediol in rice root exudates.
Key words:  Nitrification  Rice  1,9-decanediol  Biological nitrification inhibitors  Quantification

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