In this study, the effects of reduced chemical fertilizers combined with effective microbial (EM) agents application on the yield and quality of fresh corn and soil property were evaluated. The designed treatments include: no fertilizer (CK), full chemical fertilizer application according to local fertilizer application rate (CF); 60% chemical fertilizer application (RCF); 60% chemical fertilizer application combined with EM agents application (RCF+EM); 60% chemical fertilizer application combined with water (RCF+W). The results showed that fresh corn yield was higher under the RCF+EM treatment than that under the CF treatment, although the difference was not significant (P>0.05); The soluble sugar, soluble protein and vitamin C contents in fresh corn were higher under RCF+EM treatment than those under CF treatment, especially the soluble protein, which was significantly higher, with 11.79% higher than that under CF treatment (P<0.05); pH, organic matter, available nutrients and microbial carbon and nitrogen in the soil were also increased under the RCF+EM treatment compared to that under the CF treatment, and pH, available potassium and soil microbial biomass nitrogen were 20.08%, 15.81% and 59.96% higher under the RCF+EM treatment than those under the CF treatment (P<0.05). The number of fungi in the soil under RCF+EM treatment was significantly lower than that under CF treatment, while the number of bacteria and actinomycetes were significantly higher, with 153.42% and 124.17% increment respectively compared to the CF treatment. Therefore, the treatment with 60% chemical fertilizer application combined with EM agents can achieve the same yield of fresh corn as the full chemical fertilizer application treatment, meanwhile significantly improve the fresh corn quality and promote soil fertility and the number of soil microbial.