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亚油酸钠刺激多环芳烃污染土壤微生物修复的机理研究
袁 静1, 王青玲2, 侯金玉2, 张 杰1, 刘五星2, 骆永明2
1.安徽师范大学生命科学学院;2.中国科学院土壤环境与污染修复重点实验室(南京土壤研究所)
摘要:
根系分泌物在多环芳烃(PAHs)污染土壤的植物根际修复过程中发挥关键作用,但是向土壤中单独施入根系分泌物化学物质对PAHs去除的影响还少有研究。本试验通过土壤微宇宙培养试验和高通量测序技术研究了根系分泌物亚油酸钠对土壤微生物群落及PAHs降解的影响。结果发现,60 d后,添加肥料与亚油酸钠处理对土壤中PAHs的去除率为40.6%,显著高于仅施肥处理的17.4%。主坐标分析(PCoA)表明添加亚油酸钠显著改变了土壤微生物群落,土壤细菌和真菌群落组成与仅施肥处理明显分异。此外,亚油酸钠的添加还促进了PAHs降解菌如MarmoricolaStreptomycesunclassified_IntrasporangiaceaeKribbella等细菌,以及unclassified_ChaetomiaceaeMortierellaHumicola 等真菌的富集。LEFSe分析表明,StreptomycesKribbellaHumicola是添加亚油酸钠处理的主要微生物标记物,且StreptomycesKribbella相对丰度与土壤中PAHs含量呈负相关。本研究结果初步揭示了亚油酸钠强化土壤PAHs生物降解的机理。
关键词:  多环芳烃  根系分泌物  亚油酸钠  微生物群落
DOI:10.13758/j.cnki.tr.2020.05.011
分类号:X53
基金项目:国家自然科学基金项目(U1662110,41671325)和江苏省自然科学基金项目(BK20171521)资助。
Mechanism of Sodium Linoleate Stimulating Microbial Remediation of PAHs Contaminated Soil
YUAN Jing1, WANG Qing-ling2, HOU Jinyu2, ZHANG Jie1, LIU Wuxing2, LUO Yongming2
1.College of Life Sciences, Anhui Normal University;2.Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences
Abstract:
Root exudates play a key role in phytoremediation of PAHs contaminated soil, but the effects of separate root exudates on the removal of PAHs have not been studied. This experiment investigated the effects of sodium linoleate in root exudation on soil microbial community and PAHs degradation by soil microcosm culture experiment and high-throughput sequencing technology. After 60 days, the removal rates of PAHs was 40.6% for fertilizer + sodium linoleate, which were significantly higher than that of fertilization only (17.4%). Primary coordinate analysis (PCoA) indicated that the addition of sodium linoleate significantly altered soil microbial communities, and soil bacterial and fungal community compositions were significantly different from those of fertilization only. In addition, sodium linoleate amendment promoted the enrichment of PAH-degrading bacteria such as Marmoricola, Streptomyces, unclassified_Intrasporangiaceae and Kribbella, as well as the fungi unclassified_Chaetomiaceae, Mortierella and Humicola . LEFSe analysis showed that Streptomyces, Kribbella and Humicola were the main microbial markers for the addition of sodium linoleate treatment, and the relative abundance of Streptomyces and Kribbella was significantly negatively correlated with PAHs content in soil. The results of this study initially revealed the mechanism of sodium linoleate enhanced biodegradation of polycyclic aromatic hydrocarbons in soils.
Key words:  Polycyclic aromatic hydrocarbons  Root exudates  Sodium linoleate  Microbial communities

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