Abstract:A pot experiment was conducted to study the effect of plant growth-promoting rhizobacteria (PGPR) on rice growth after added into seeding substrate, in which five PGPR were selected, included LY5 (Bacillus subtilis), LY11 (Bacillus amyloliquefaciens), X2 (Pseudomonas moraviensis), X3 (Bacillus safensis) and X8 (Pseudomonas chlororaphis), and mixed with an efficient seedling substrate, respectively. The results showed that, compared with the CK (seedling substrate without PGPR added), LY11 was the best in promoting the growth and metabolic activity of rice seedlings, PGRP treatments significantly increased the aboveground biomass and strong seedlings index by 17.24%-31.19% and 11.37%-23.28%, respectively, significantly increased the volume, length and activity of rice roots and improved the morphology and structure of rice roots, significantly increased the uptake of nitrogen, phosphorus and potassium, the transport coefficients of nitrogen and potassium, and GS and SPS activities of rice seedlings. In conclusion, the application of PGPR to rice seedling substrate is benefit to the growth and activity of rice seedlings and can produce high quality rice seedling substrate.