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多菌灵降解菌djl-6和啶虫脒降解菌D-2固体菌剂的研发
徐铭阳, 卢家森, 孟献雨, 林泽彬, 何健, 黄星
南京农业大学生命科学学院, 南京 210095
摘要:
以蚯蚓粪肥、猪粪肥、鸡粪肥、秸秆肥、米糠、泥炭以及花生壳粉作为载体,以多菌灵降解菌Rhodococcus qingshengii djl-6和啶虫脒降解菌Pigmentiphaga sp.D-2为材料,开展了固体菌剂的研发。接种菌株djl-6及D-2后,各载体均能够促进供试菌株的生长。以蚯蚓粪肥作为载体时,菌株djl-6、D-2有效菌数释放率最高,分别为100.40%和82.03%。在保存120 d时,除花生壳粉外,其余各载体中的活菌数均高于107 cfu/g;以蚯蚓粪为载体的djl-6菌剂活菌数达到了7.00×108 cfu/g,以猪粪为载体的D-2菌剂活菌数达到了4.29×108 cfu/g。固体菌剂保存30 d后进行土壤农药残留降解试验,以蚯蚓粪为载体的djl-6菌剂对5 mg/kg的多菌灵降解率为94.30%,以蚯蚓粪为载体的D-2菌剂对10 mg/kg的啶虫脒降解率为81.87%。研究结果表明,以蚯蚓粪为载体的固体农残降解菌剂活菌数高、保存期长,具有较好的应用前景。
关键词:  多菌灵降解菌djl-6  啶虫脒降解菌D-2  固体菌剂
DOI:10.13758/j.cnki.tr.2021.02.016
分类号:X172
基金项目:国家重点研发计划项目(2016YFD0801102)、江苏省农业科技自主创新资金项目(CX(18)1005)和江苏省高等学校大学生实践创新训练计划项目(201810307012)资助。
Study of Solid Bacterial Agents for Carbendazim-degrading Strain djl-6 and Acetamiprid-degrading Strain D-2
XU Mingyang, LU Jiasen, MENG Xianyu, LIN Zebin, HE Jian, HUANG Xing
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China
Abstract:
In this study, earthworm manure, pig manure, chicken manure, straw manure, rice bran, peat, and peanut shell powder were used as carriers. The carbendazim-degrading bacteria Rhodococcus qingshengii djl-6 and the acetamiprid-degrading bacteria Pigmentiphaga sp. D-2 were used as materials. After being inoculated the strains djl-6 and D-2, all carriers could promote the growth of the tested strains. When earthworm manure was used as the carrier, the effective release rates of strains djl-6 and D-2 were the highest, reaching 100.40% and 82.03% respectively. When stored for 120 d, the number of viable bacteria in each carrier except peanut shell powder was higher than 107 cfu/g. The live bacterial count of djl-6 bacterial agent using earthworm manure as a carrier reached 7.00×108 cfu/g, and the live bacterial count of D-2 bacterial agent using pig manure as a carrier reached 4.29×108 cfu/g. After the solid bacterial agent was stored for 30 d, the soil pesticide residue degradation test was performed. The djl-6 bacteria agent with earthworm manure as a carrier degraded 94.30% of carbendazim at 5 mg/kg, and the D-2 bacteria with earthworm manure as a carrier degraded 81.87% of carbendazim at 10 mg/kg. The results of this study indicated that the solid pesticide residue degrading bacterial agent using earthworm manure as a carrier has a high number of live bacteria and a long shelf life, and has a good application prospect.
Key words:  Carbendazim-degrading strain djl-6  Acetamiprid-degrading strain D-2  Solid bacterial agent

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