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微生物菌种筛选技术方法研究进展
荣楠1, 李备2,3, 唐昊冶1, 林先贵1, 冯有智1
1.中国科学院南京土壤研究所, 南京 210008;2.中国科学院长春光学精密机械与物理研究所, 长春 130033;3.长春长光辰英生物科学仪器有限公司, 长春 130033
摘要:
微生物菌种筛选能帮助人类全面认识地球环境中微生物群落的组成与功能,具有重要的科研价值、生态环境与社会经济效益。传统的菌种筛选方法过程繁琐、费时费力。近期新兴的现代菌种筛选方法因其操作简便快捷而成为当前的国际研究热点。本文综合国内外相关研究进展,重点介绍了传统菌种筛选方法和以基于光学镊子结合拉曼光谱的细胞分选技术、荧光活化细胞分选法、基于激光诱导向前转移技术的细胞分选技术、基于原位培养的细胞分离技术为代表的现代菌种筛选方法的优缺点,并进一步对微生物菌种筛选技术的未来发展做出了初步的展望。
关键词:  微生物  菌种筛选  平板筛选  光学镊子  拉曼光谱  流式细胞仪  激光诱导向前转移技术  原位培养
DOI:10.13758/j.cnki.tr.2021.02.003
分类号:X171;X172
基金项目:国家自然科学基金项目(41771294)资助。
Advances in Strain Isolating Technique and Method for Microorganisms
RONG Nan1, LI Bei2,3, TANG Haoye1, LIN Xiangui1, FENG Youzhi1
1.Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;2.Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China;3.HOOKE Instruments Ltd., Changchun 130033, China
Abstract:
Microbial strain screening can greatly help understand the roles of Earth's microbiome, and thus has the high scientific merit, as well as the associated ecological and social economic benefits. Although the traditional methods are feasible, their processes for screening strains are tedious and time consuming. Alternatively, the modern screening techniques recently have become a hot spot in worldwide due to the features of convenience and high throughput etc. In this paper, current domestic and international research advances on microbial strain screening, such as laser tweezers and Raman spectroscopy, flow cytometry, laser-induced forward transfer and in situ cultivation were briefly introduced, by comparing their advantages and disadvantages against those of traditional methods. Then the prospects of these techniques and methods in future were forecasted.
Key words:  Microorganism  Strain screening  Plate screening  Laser tweezers  Raman spectroscopy  Flow cytometer  Laser-induced forward transfer  In situ cultivation

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