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木质素与蚯蚓对黑麦草生物量及土壤微生物群落的影响
孙月,潘彦硕,曾军,吴宇澄,林先贵
1.中国科学院土壤环境与污染修复重点实验室(南京土壤研究所), 南京 210008;2.中国科学院大学, 北京 100049;3.西北农林科技大学资源环境学院, 陕西杨凌 712100
摘要:
采集农田土壤,设置添加木质素和蚯蚓不同组合处理的黑麦草盆栽试验,在测定植物生物量的基础上,结合定量PCR、高通量测序等方法,研究木质素和蚯蚓及其联合作用对土壤细菌、真菌群落的影响。结果显示:木质素显著降低了黑麦草的生物量,而蚯蚓能够缓解木质素的抑制作用,促进黑麦草生长。木质素明显抑制了土壤细菌、真菌数量,并改变群落结构,以Sphingomonadaceae、Methylophilaceae为标志的降解细菌和担子菌等潜在降解真菌显著富集;单一加入蚯蚓对土壤微生物的影响相对较小,木质素和蚯蚓组合处理显著提高细菌、真菌数量,改变上述降解微生物的丰度。这些结果表明,木质素降低土壤微生物数量,并可能通过其自身降解释放的酚类化感物质抑制植物生长,对土壤生态系统健康产生不利影响;蚯蚓与木质素联合处理影响土壤中木质素降解微生物组成,微生物数量与植物生物量均表现增加趋势,蚯蚓有利于土壤中木质素的微生物代谢,并为植物生长提供更多的养分。本研究为农田生态系统木质素资源化利用提供了科学依据。
关键词:  木质素  蚯蚓  黑麦草  土壤微生物
DOI:10.13758/j.cnki.tr.2021.02.014
分类号:X172;X173
基金项目:国家自然科学基金项目(41977132,41671266)资助。
Effects of Lignin and Earthworm on Ryegrass Biomass and Soil Microbial Community
SUN Yue1,2, PAN Yanshuo3,4, ZENG Jun1, WU Yucheng1,2, LIN Xiangui1,2
1.Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.College of Natural Resources and Environment, Northwest Agriculture &4.Forestry University, Yangling, Shaanxi 712100, China
Abstract:
In order to investigate the effects of lignin and earthworms and their combined effects on plant biomass and soil microorganisms, farmland soils were collected, and ryegrass pot experiments with different combinations of lignin and earthworms were setup. Based on the determination of biomass, combined with methods such as quantitative PCR and high-throughput sequencing, the effects of lignin and earthworms on soil bacterial and fungal communities were studied. The results showed that lignin significantly reduced ryegrass biomass, while earthworms could alleviate the inhibitory effect of lignin and promote ryegrass biomass. Lignin significantly inhibited the numbers of soil bacteria and fungi, and changed the community structures, and potential degrading bacteria such as Sphingomonadaceae and Methylophilaceae were significantly enriched. The effect of adding earthworms on soil microorganisms was relatively small, and the combined treatment of lignin and earthworms significantly increased the copy numbers of bacteria and fungi and changed the abundance of the aforementioned degrading microorganisms. These results indicated that lignin reduced the copy number of soil microorganisms, and may inhibit plant growth through phenolic allelopathic substances released by its own degradation, which adversely affected the health of soil ecosystems. The combined treatment of earthworm and lignin affected the composition of lignin-degrading microorganisms in the soil, and both microorganism population and plant biomass tended to increase, suggesting that earthworm is beneficial to the microbial metabolism of lignin and lignin-derived phenolic compounds in the soil. This study provides a scientific basis for the utilization of lignin resources in farmland ecosystems.
Key words:  Lignin  Earthworm  Ryegrass  Soil microorganisms

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