Flue-cured tobacco has a wide range of ecological adaptability and is sensitive to the changes of ecological conditions. The flue-cured tobacco regionalization can provide references for the production and development of high-quality flue-cured tobacco in China. This paper described the research status of flue-cured tobacco regionalization in China from the following aspects: integrated method of comprehensive indexes for tobacco regionalization, tobacco regionalization at the scales of country, region, province, city and county. At present, the integrated methods of comprehensive indexes for tobacco regionalization mainly include cluster analysis and index summary, etc. Tobacco regionalization, at large scale (at country and region scales), plays an important role in guiding the cultivation of flue-cured tobacco in China, but at the small scale (such as at city and county scales), it cannot meet the demand of precision purchase of flue-cured tobacco raw materials of current cigarette industry enterprises in China. In the future, in order to provide the basis for the cultivation and quality selection of flue-cured tobacco, it is necessary to strengthen the research on the influence of ecological factors on the quality of flue-cured tobacco, improve the accuracy of tobacco regionalization at small scales, verify the accuracy of tobacco regionalization, and realize the dynamic regionalization of flue-cured tobacco.