稻草覆盖和香根草篱措施下坡耕地红壤细菌群落结构特征及其影响因素
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S154.2

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国家自然科学基金项目(41301235)和江西省重点研发计划项目(20181BBF68009)资助。


Bacterial Community Structures and Influential Factors Under Straw Mulching and Vetiver Grass Hedgerows in Sloping Land of Red Soil
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    摘要:

    为了揭示长期稻草覆盖和香根草篱措施下坡耕地红壤细菌群落结构特征,分析影响红壤细菌群落变化的主控环境因子,试验依托红壤坡地稻草覆盖和香根草篱等措施的水保效果长期定位试验,采用高通量测序技术,研究花生常规等高种植(CK)条件下,配合香根草篱(H)、稻草覆盖(M)和稻草覆盖+香根草篱(HM)措施对土壤细菌群落结构的影响,并分析细菌群落组成与土壤性状的相关性。结果表明:①与CK处理相比,H、M和HM处理土壤细菌丰富度和Shannon多样性指数均有增加,香根草篱和稻草覆盖措施均是增加土壤细菌丰富度的影响因素,稻草覆盖措施是增加土壤细菌多样性的主要影响因素,HM处理特有种细菌数量最多。②水保措施对土壤细菌群落结构产生影响,相比CK处理,M处理显著增加了变形菌门(Proteobacteria)细菌的相对丰度5.1%,降低了绿弯菌门(Chloroflexi)细菌的相对丰度,HM处理变形菌门和拟杆菌门(Bacteroidetes)细菌的相对丰度分别显著增加5.4%和1.2%,而H处理土壤细菌群落组成与CK处理间无显著差异。③主成分分析结果显示,有稻草覆盖处理(M和HM)的细菌群落结构相似,但与CK和H处理间细菌群落结构差异较大;典范对应分析表明,木聚糖酶、N-乙酰-β-D-氨基葡萄糖苷酶活性与细菌群落结构组成密切相关,土壤有机碳和硝态氮含量是影响细菌群落结构的主要环境因子。

    Abstract:

    In this paper, bacterial community structures were studied in the slope land of red soil under straw mulching and vetiver grass hedgerows and their relations with soil environmental factors were also investigated in order to provide the basis for soil microbial diversity maintenance and proper land management. Soil samples were collected from an experimental field (since 2009) with different soil and water conservation measures, including peanut contour farming (CK), peanuts + vetiver grass hedgerows, peanuts + rice straw mulching (M) and peanuts + vetiver grass hedgerows + rice straw mulching (HM), the abundances, diversities and compositions of bacterial communities were analyzed by high-throughput sequencing of 16S rRNA genes (Illumina Miseq platform), and their relations with soil environmental factors were analyzed by using canonical correspondence analysis (CCA) and factor analysis. The results showed that H, M and HM increased soil microbial richness and Shannon index compared with CK, H and M dominated microbial richness, M dominated bacterial diversity, and HM had the highest number of endemic species of bacteria. Soil and water conservation measures affected soil bacterial community structures, compared with CK, M significantly increased the relative abundance of Proteobacteria by 5.1% and decreased the relative abundance of Chloroflexi, while HM significantly increased the relative abundance of Proteobacteria and Bacteroidetes by 5.4% and 1.2% respectively. Principal component analysis (PCA) showed that bacterial community structures were similar between M and HM, but significantly different with those of CK and H. CCA analysis showed that the activities of xylanse and N-acetyl-β-D-glucosaminidase were closely related to bacterial community composition, and the contents of soil TOC and NO3--N were main environmental factors associated with bacterial community.

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成艳红,王馨悦,林小兵,黄尚书,张昆,何绍浪,王斌强,万长艳,黄欠如.稻草覆盖和香根草篱措施下坡耕地红壤细菌群落结构特征及其影响因素[J].土壤,2021,53(3):522-528. CHENG Yanhong, WANG Xinyue, LIN Xiaobing, HUANG Shangshu, ZHANG Kun, HE Shaolang, WANG Binqiang, WAN Changyan, HUANG Qianru. Bacterial Community Structures and Influential Factors Under Straw Mulching and Vetiver Grass Hedgerows in Sloping Land of Red Soil[J]. Soils,2021,53(3):522-528

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  • 收稿日期:2020-05-25
  • 最后修改日期:2020-07-15
  • 录用日期:2020-07-21
  • 在线发布日期: 2021-06-18
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