In order to realize soil conservation and sustainable utilization of sloping farmland, the main nutrient transportation characteristics of red slope soil in flue-cured tobacco growing season in Yunnan Province were explored. The results showed that soil pH, cation exchange capacity (CEC) and contents of main nutrients decreased linearly while soil bulk density increased with the increase of soil depth. The contents of alkali-hydrolyzed nitrogen, available phosphorus and available potassium varied little under soil depth of 30 cm, and with the growth period of flue-cured tobacco, soil chemical properties of profile were improved to varying degrees. The main nutrient contents of ridge and alluvial soils decreased from top to bottom of the slope, and the decrease was smaller in ridge soil than in alluvial one, the contents of organic matter in same size aggregates under same slope position condition were higher in ridge soil than in alluvial one. The tobacco plant growth characteristics in red soil slope farmland were lower slope>middle slope>upper slope. In conclusion, the downward potential is weak for major soil nutrients below the tilled layer (30 cm) in Yunnan red soil slope farmland, and the slope position factor will accelerate the impoverishment of upper slope soil.