The distribution and diversity of arbuscular mycorrhizal (AM) fungi in Wuchuan mercury (Hg) mining area of Guizhou Province were studied, and the results showed that soil Hg contents in three samples ranged from 94.1 to 268.0 mg/kg, which were much higher than the national guideline concentration. Hg pollution was extremely serious. With the increase of Hg concentration, AM inoculation, the density of spores decreased significantly, while the abundance, diversity and evenness were decreased insignificantly. However, some AM fungi species with high tolerance to Hg contamination still had high distribution. The relative abundance of Funneliformis was also found high in all the five samples, and the relative abundance of Glomus in Pyracantha rhizosphere increased with the increase of Hg concentration. Therefore, the combination of Pyracantha- Funneliformis spp. or Pyracantha-Glomus spp. maybe used in the remediation of Hg contamination.