In order to explore the relationship between land-use change and soil physicochemical and biological properties under the background of the integration of agriculture and tourism, and clarify the impact of land-use change from paddy fields to economic forest lands on soil properties, several representative soil profiles in Shaoxing City, Zhejiang Province, were selected and layer samples were collected, and the evolution of soil properties, morphology and microbial characteristics were studied. The result showed after the land-use transformation from paddy field to forest land, the groundwater level decreased obviously, soil compactibility of surface layer increased by 6.79 times while bulk density increased by 33.7%. Soil color brightness and chromaticity increased, soil structure size increased. The contents of total and free iron increased by 7.44% and 8.70%, however active and ferrous iron decreased by 18.20% and 91.56%, respectively. Moreover, total amount of phospholipid fatty acids of soil microorganisms decreased by 44.57%, the ratio of aerobic to anaerobic bacteria increased by 92.6%, and the ratio of Gram-negative bacteria to Gram-positive bacteria decreased by 45.3%. In conclusion, the land-use change from paddy field to forest land can obviously change soil physiochemical properties under the background of the integration of agriculture and tourism, which has adverse effects on the growth of vegetation and microorganisms and unconducive to the sustainable utilization of soil.