The dissimilatory nitrate reduction to ammonium (DNRA) is a vital pathway for soil nitrogen transformation in ecosystems. Clarifying the impact of environmental factors on soil DNRA rates is urgent and significant. In this study, 246 experimental observation data were collected to investigate the variation ranges of DNRA rates and their influencing factors in different ecosystems in the subtropical regions using meta-analysis. The results showed that latitude had no significant effect on subtropical soil DNRA rate. The increase in mean annual temperature (MAT), pH, total nitrogen (TN), SOC/NO- 3-N, Fe(II), cation exchange capacity (CEC), easily oxidizable organic carbon (EOC), available phosphorus (AP), and soil clay content promoted DNRA rate. However, the increase of mean annual precipitation (MAP) and soil NH+ 4-N content inhibited soil DNRA. In addition, the effects of environmental factors on DNRA rates were complicated in different ecosystems. Structural equation model analysis revealed that SOC/NO- 3-N was the main driving factor of DNRA, and MAP, MAT, pH, and NH+ 4-N also had a significant influence on soil DNRA.