In order to master the spatial distribution characteristics of soil nutrients and reasonable soil sampling in coniferous and broad-leaved mixed forests of subtropical mountains, in this study, Pinus massoniana-Castanopsis eyrei mixed forest (PCF), Pinus massoniana pure forest (PF, control) in central Guizhou were selected as research objects, the adjacent grid method was used to investigate and collect soil samples, and the spatial distribution characteristics of soil nutrients were analyzed by using classical statistics and geostatistical analysis methods. The results showed as follows:1)Significant differences were found in soil pH, organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), available nitrogen (AN), available phosphorus (AP) and available potassium (AK) between PF and PCF (P<0.05). 2)Compared with PF, the spatial heterogeneity of all soil nutrients in PCF was more obvious. 3)Cross-validation analysis showed that the appropriate sampling number and sampling area of PCF and PF were 24, 600 m² and 17, 425 m². The results of kriging interpolation showed that soil nutrients of subtropical mountainous coniferous and broad mixed forests were characterized by flaky or patchy distribution with a poor uniformity.