Oral ingestion is an important route of human exposed to soil polycyclic aromatic hydrocarbons (PAHs). Effective assessment of the bioaccessibility of PAHs is an important component of a comprehensive assessment of the human health risk of PAHs in soil. However, there are many in vitro gastrointestinal simulation methods to evaluate the bioaccessibility of soil PAHs, and there is still no uniform standard in China as well as abroad. The development of human health risk assessment of soil PAHs has been hampered by the large variation in the results of different in vitro simulation methods. Thus, this paper reviewed various in vitro gastrointestinal simulation methods and the related advances for PAHs bioavailability determination, and suggested that future research on the construction of standard methods for PAHs bioaccessibility determination in soils should be based on three aspects:physiological conditions, selection of adsorbent materials, and validation of in vivo models.