To explore the effects of selenium (Se) application on total, inorganic and organic Se contents in wheat grains, with no Se application as the control group, a meta-analysis was used to comprehensively analyze the effects of factors such as Se source, application season, frequency, amount, and method of Se fertilizers on wheat Se absorption. The results showed that Se application can increase total Se content in wheat grains, with the highest effect of selenate (2.53), but there was no significant difference between different Se sources. The response of Se uptake in wheat varies at different seasons, with better effects observed during the late-growth stage (booting stage filling stage), especially during the flowering stage (2.16) and grouting stage (2.17). The maximum effect (>2.00) on inorganic and organic Se contents in wheat grains was observed when Se application rate was 60-100 g/hm². The promotion effect of soil application(up to 3.95) on total Se content in wheat grains is significantly higher than that of foliar spraying, but foliar spraying is more conducive to the accumulation of inorganic Se in grains. Applying Se less than or equal to 2 times has a significant promoting effect on Se content in wheat grains, but the promoting effect decreases as the frequency increases. In practical production, various factors should be comprehensively considered to select selenate, apply Se in the later stage of growth, reduce the frequency of Se application (≤2 times), and adjust the amount of Se application appropriately (≤100 g/hm²) to maximize the enrichment of Se in wheat.