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盐分胁迫下控释尿素配施腐植酸对棉花幼苗生长和抗氧化系统的影响
郭新送1,2, 苏秀荣3, 范仲卿1,2, 于晓东1,2, 宋挚1,2, 朱福军1,2, 丁方军1,2,4
1.农业部腐植酸类肥料重点实验室;2.山东省腐植酸高效利用示范工程技术研究中心,山东农大肥业科技有限公司;3.山东农业大学化学学院;4.土肥资源高效利用国家工程实验室(泰安)
摘要:
为探究盐分胁迫下,控释尿素配施腐植酸对棉花幼苗生长特性和棉花植株体内抗氧化系统的影响,本试验以滨海盐化潮土(全盐含量4.5g/kg)为供试土壤,设置不施氮肥,普通尿素、控释尿素、普通尿素+腐植酸、控释尿素+腐植酸5个处理进行棉花盆栽试验。采集盆栽棉花植株测定生物产量、叶片叶绿素含量、植株叶片及根系抗氧化物酶(SOD、POD、CAT)活性和过氧化物质(MDA、O2? ?)含量。结果表明,盐分胁迫下,控释尿素配施腐植酸有利于促进棉花幼苗期生物量的累积,较其它处理的棉花地上部鲜、干重分别提高13.46~69.46%和12.64%~82.23%,差异显著(p<0.05);普通尿素和控释尿素配施腐植酸处理的叶绿素a含量分别提高5.37%和11.84%,其中控释尿素配施腐植酸处理显著高于其它处理;两种尿素配施腐植酸处理的棉花叶片和根系内SOD活性提高3.41%~42.84%,POD活性提高了4.376%~37.87%,CAT活性分别提高了5.53%~64.61%,其中控释尿素配施腐植酸的SOD、POD以及CAT活性显著高于未施用腐植酸处理。盐分胁迫下,控释尿素和腐植酸具有协同抗逆作用,其通过提高棉花体内抗氧化物酶的活性和降低植物体内过氧化物质的累积量来提高对盐分胁迫的抗逆能力。
关键词:  盐分胁迫 控释尿素 腐植酸 叶绿素 抗氧化酶 过氧化物质
DOI:
分类号:S158.3; S154.3
基金项目:十三五国家重点研发计划“功能性缓控释肥研制与应用” (2017YFD0200706)。
Effects of Combined Application of controlled-release urea and humic acid on growth and antioxidant system in Cotton Seedlings under Salt Stress
Guo Xinsong1,2, Su Xiurong3, Fan ZHongqing1,2, Yu Xiaodong1,2, Song ZHi1,2, Zhu Fujun1,2, Ding Fangjun1,2,4
1.Key Laboratory of Humic Acid Fertilizer, Ministry of Agriculture;2.Engineering Technology Research Center of Shandong Province, Efficient Utilization of Humic Acid, Shandong Agricultural University Fertilizer Science Tech. Co. Ltd.;3.College of Chemistry, Shandong Agricultural University;4.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Taian
Abstract:
In order to explore the effects of combined application controlled-release urea and humic acid on the growth characteristics and the antioxidant system in cotton seedlings under salt stress. A cotton pot experiment was conducted by five treatments with coastal saline soil (4.5g/kg of total salt), which include no nitrogen fertilizer, common urea, controlled-release urea, common urea + humic acid, controlled-release urea + humic acid treatments. Potted cotton plants and checked the cotton biomass, chlorophyll content, antioxidant enzymes (SOD, POD, CAT) activity and peroxide (MDA, O2-) content in leaves and roots. The results showed that under salt stress, controlled-release urea + humic acid treatment promoted the cotton seedling’s biomass, compared with other treatments, the aboveground fresh and dry weight of cotton increased by 13.46-69.46% and 12.64-82.23%respectively, with significant difference (p < 0.05). The chlorophyll a content in cotton leaves increased by 5.37% and 11.84% of common urea + humic acid and controlled-release urea + humic acid treatments, respectively. The chlorophyll a content of controlled-release urea + humic acid treatment was significantly higher than that in other treatments. Common urea + humic acid and controlled-release urea + humic acid treatments promoted the antioxidant enzymes activity of cotton leaves and roots, SOD activity increased by 3.41%~42.84%, POD activity increased by 4.376%~37.87% and CAT activity increased by 5.53%~64.61%, respectively. The activities of SOD, POD and CAT of controlled-release urea + humic acid treatment were significantly higher than the other treatments. Combined application controlled-release urea and humic acid had synergistic stress resistance under salt stress, which could improve the stress resistance of cotton by increasing the activity of antioxidant enzymes and reducing the accumulation of peroxides in plants.
Key words:  salt stress  controlled-release urea  humic acid  chlorophyll  antioxidant enzyme  per oxidation substance

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