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外源NO缓解花生幼苗盐胁迫伤害的生理机制
朱营营, 董元杰, 张倩, 万勇善, 刘风珍
山东农业大学
摘要:
花生是我国重要的油料和饲料作物,但盐渍化环境下出苗难、保苗难。为研究外源添加不同浓度硝普钠(一氧化氮供体,SNP)缓解花生幼苗盐害的效应及其生理机制,明确SNP的最适添加浓度;本文以花生品种“山花11号”为供试材料,SNP为外源NO供体进行液培试验,研究不同浓度SNP(50、100、250、300 μmol L-1)对100 mmol L-1 NaCl胁迫下花生幼苗生长及生理特性的影响。结果表明,盐胁迫显著抑制花生幼苗生长,添加SNP后能有效提高花生苗期耐盐性,且SNP对花生盐害的缓解作用随着浓度的增加先升后降,其中250μmol L-1 SNP对花生幼苗盐胁迫的缓解作用最强。与CK相比,Hoagland +100 mM NaCl+250μM SNP处理的株高、鲜干重分别增加了26.94%、34.73%、36.36%;叶片和根系SOD活性分别提升了3.39%、1.66%,POD活性分别提升了32.14%、51.88%,CAT活性分别提升了180.39%、88.24%;脯氨酸含量提升了281.28%;花生体内抗氧化酶活性和渗透调节物质含量的提高,减少了丙二醛(MDA)和活性氧(ROS)的积累,减轻膜脂过氧化损伤,叶片和根系MDA含量分别降低了23.82%、30.98%,O2??产生速率分别降低了54.68%、54.23%,H2O2含量分别降低了13.98%、21.12%;显著增加了叶片叶绿素总含量21.93%,花生根系活力提高了22.45%,抑制根系对Na+的吸收,幼苗体内K+/Na+提高了118.99%,显著降低了盐胁迫危害。上述结果显示,SNP对花生盐害的缓解作用随SNP浓度的增加先升高后降低,存在明显的剂量效应,其中250μM SNP对花生幼苗盐胁迫的缓解效果最优。
关键词:  一氧化氮  花生  盐胁迫  生理特性
DOI:
分类号:S311
基金项目:山东省自然科学基金(ZR2017MD010),山东省农业重大应用技术创新项目(SD2019ZZ021)和泰山学者种业创新团队项目(2014)
Physiological Mechanisms of Exogenous NO to Alleviate Salt Stress Damage of Peanut Seedlings
zhu yingying, dongyuanjie, zhangqian, wan yongshan, liu fengzhen
shandong agricultural university
Abstract:
Peanut is an important oil and feed crop in China, but it is difficult to produce seedlings and protect seedlings in a salinized environment. In order to study the effects of exogenous addition of different concentrations of sodium nitroprusside (nitrogen monoxide donor, SNP) on the salt damage of peanut seedlings and its physiological mechanism, the optimal concentration of SNP was determined. In this paper, the peanut variety “Hanhua 11” was used as the test material, and the SNP was the external NO donor for liquid culture test. The effects of different concentrations of SNP (50, 100, 250, 300 μmol L-1) on the growth and physiological characteristics of peanut seedlings under 100 mmol L-1 NaCl stress were studied. The results showed that salt stress significantly inhibited the growth of peanut seedlings, and the addition of SNP could effectively improve the salt tolerance of peanut seedlings. Moreover, the alleviation effect of SNP on peanut salt increased first and then decreased with the increase of concentration. Among them, 250μmol L-1 SNP had the strongest alleviation effect on salt stress of peanut seedlings. Compared with CK, the plant height and fresh dry weight of Hoagland+100 mM NaCl+250μM SNP increased by 26.94%, 34.73% and 36.36%, respectively; SOD activity of leaves and roots increased by 3.39% and 1.66%, POD activity increased by 32.14% and 51.88%, and CAT activity increased by 180.39% and 88.24%, respectively; proline content increased by 281.28%. The increase of antioxidant enzyme activity and osmotic substance content in peanut reduces the accumulation of malondialdehyde (MDA) and reactive oxygen species (ROS), and reduces the damage of membrane lipid peroxidation; leaf and root MDA content decreased by 23.82%, 30.98%, O2?? production rate decreased by 54.68%, 54.23%,H2O2 content decreased by 13.98 %, 21.12%, respectively; significantly increased the total chlorophyll content of the leaves by 21.93%, the peanut root activity increased by 22.45%, inhibited the absorption of Na+ by the roots, and the K+/Na+ of the seedlings increased by 118.99%, which significantly reduced the salt stress hazard. The above results showed that the alleviation effect of SNP on peanut salt decreased first and then decreased with the increase of SNP concentration, and there was a significant dose effect. Among them, 250μM SNP had the best effect on the salt stress of peanut seedlings.
Key words:  Nitric oxide  peanut  salt stress  Physiological characteristics

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